HPV Cocktail Broad Spectrum
The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.
CAMVIR-1 is also available as a monoclonal and can be used separately for phenotyping HPV-16. Other HPV isotypes may also be reactive with the Broad Spectrum HPV antibody, but have not been tested.
| Ordering Information |
Catalog Number |
Volume |
| Concentrate |
CM 177 |
BK, CK |
| Predilute |
PM 177 |
AA |
| intelliPATH™ |
N/A |
N/A |
| VP Echelon |
N/A |
N/A |
| A / AK = 0.1 ml |
B / BK = 0.5 ml |
C / CK = 1.0 ml |
G5 = 5.0 ml |
AA = 6.0 ml |
H / G25 = 25 ml |
L = 100 ml |
