ER
Clone SP1 has been established to work in formalin-fixed, paraffinembedded tissues. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (tamoxifen) therapy. Staining patterns observed include: ER+ and PR+, ER+ and PR-, ER- and PR+, and ER- and PR-. The SP1 clone is a high affinity rabbit monoclonal. An in-house study showed that SP1 clone had 5 times more binding capacity than ER clone 6F11 and 10 times more binding capacity than ER clone 1D5. Studies also have shown that the SP1 clone is superior to 1D5 in predicting survival. Excellent results can be achieved with antigen retrieval at lower temperatures such as 80 or 95 degrees Celsius. The robustness of SP1 is further proved by the fact that in some instances staining can be obtained even without antigen retrieval. Thus, SP1 may prove of great value in the assessment of ER status in human breast cancer.
| Ordering Information |
Catalog Number |
Volume |
| Concentrate |
N/A |
N/A |
| Predilute |
PRM 301 |
AA |
| intelliPATH™ |
N/A |
N/A |
| VP Echelon |
N/A |
N/A |
| A / AK = 0.1 ml |
B / BK = 0.5 ml |
C / CK = 1.0 ml |
G5 = 5.0 ml |
AA = 6.0 ml |
H / G25 = 25 ml |
L = 100 ml |
