GI

MUC-1

Breast cancer stained with MUC-1 antibody

MUC-1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. The MUC-1 mucin (Cancer Antigen 15-3) is secreted from tumor cells. The apoprotein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. Abnormal overexpression of MUC-1 in cancer cells is thought to contribute to their aggressive growth, but molecular mechanisms associated with this effect are still unclear. MUC-1 stains cell membranes, but also the cytoplasm of most epithelial cell types. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over-expressed abundantly in >90% breast carcinomas and metastases. As an exception, mucinous carcinomas are significantly less MUC-1 reactive. Aberrant cytoplasmic and membranous localization of MUC-1 expression has been associated with poor patient outcome. Adenocarcinomas are generally positive and squamous carcinomas and nonepithelial malignancies negative. High grade prostate carcinomas were negative, in contrast to low grade ones. Bladder and kidney cancers were either strongly positive or negative. Hepatocellular carcinomas are negative, but cholangiogenic carcinomas are positive.

Specifications  
Intended Use
Source
Species Reactivity ,
Clone
Isotype
Antigen
Cellular Localization ,
Positive Control
Normal Tissue , , , ,
Abnormal Tissue , , , ,
Ordering Information Catalog Number Volume
Concentrate CM 319 B
Predilute PM 319 AA
intelliPATH™ N/A N/A
VP Echelon N/A N/A
A / AK = 0.1 ml B / BK = 0.5 ml C / CK = 1.0 ml G5 = 5.0 ml AA = 6.0 ml H / G25 = 25 ml L = 100 ml
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